Enexpand Long Template Pcr System

Enexpand Long Template Pcr System - Expand Long Template PCR System Deoxynucleoside triphosphate DNA deoxynucleotidyltransferase E C 2 7 7 7 Cat No 11 681 834 001 150 U Cat No 11 681 842 001 720 U 2 360 U Cat No 11 759 060 001 3 600 U 10 360 U Version February 2005 Store the kit at 15 to 25 C 1 What this Product Does Number of Tests The kit is designed for

Buffers Always thaw and equilibrate all Expand Long Template PCR System buffers at 37 56 C before use In addition buffers should be vortexed thoroughly When crystals have formed incubate at 37 56 C until they are dissolved After this step do not place the buffers on ice After pipetting the last reaction component start the

Enexpand Long Template Pcr System

Enexpand Long Template Pcr System

Features and Benefits: Expand Long Template PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages or cosmids, and eukaryotic-gene cloning and analysis. • Amplify longer templates: This 3-buffer set amplifies products from 5 to 20kb.

Expand Long Template PCR System is optimized to efficiently amplify large genomic DNA fragments approximately 20 kb long The system is composed of a special enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture is

Expand Long Template PCR System Protocol Troubleshooting

Expand Long Template PCR System Deoxynucleoside triphosphate DNA deoxynucleotidyltransferase E C 2 7 7 7 Cat No 11 681 834 001 150 U Cat No 11 681 842 001 720 U 2 360 U Cat No 11 759 060 001 3 600 U 10 360 U Version June 2010 Store the kit at 15 to 25 C 1 What this Product Does Number of Tests The kit is designed for

Expand Long Template Pcr System Roche Protocol

General description Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product from genomic DNA Expand Long Template PCR System allows amplification of DNA fragments up to 20kb

Expand Long Template PCR System

Expand Long Template PCR System is ideal for genome mapping and sequencing contig construction characterization of cloned sequences in lambda phages or cosmids and eukaryotic gene cloning and analysis Amplify longer templates This 3 buffer set amplifies products from 5 to 20kb

Expand Long Template Pcr System Printable Word Searches

Each lot of Expand Long Template PCR System is func tion tested in PCR Routinely Expand Long Template PCR is performed using human genomic DNA and specific primers for a 9 kb 12 kb and 15 kb fragment References 1 Hopfner K P et al 1999 PNAS 96 3600 3605 2 Frey B and Suppmann B 1995 BIOCHEMICA 2 8 9

Others Expand High Fidelity PCR System Expand High Fidelity PCR System For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates.

ROCHE Expand Long Template PCR System Thomas Scientific

Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity This powerful polymerase mixture produces a high yield of PCR product from genomic DNA Expand Long Template PCR System allows amplification of DNA fragments up to 20kb from human genomic

Enexpand Long Template Pcr System

Each lot of Expand Long Template PCR System is func tion tested in PCR Routinely Expand Long Template PCR is performed using human genomic DNA and specific primers for a 9 kb 12 kb and 15 kb fragment References 1 Hopfner K P et al 1999 PNAS 96 3600 3605 2 Frey B and Suppmann B 1995 BIOCHEMICA 2 8 9

Buffers Always thaw and equilibrate all Expand Long Template PCR System buffers at 37 56 C before use In addition buffers should be vortexed thoroughly When crystals have formed incubate at 37 56 C until they are dissolved After this step do not place the buffers on ice After pipetting the last reaction component start the

Polymerase Chain Reaction PCR Steps Types And Applications